Phagocytic activity of alveolar macrophages toward polystyrene latex microspheres and PLGA microspheres loaded with anti-tuberculosis agent

摘要

Phagocytosis of alveolar macrophages (M phi s) toward poly(lactic-co-glycolic acid) (PLGA) microspheres (MS) loaded with the anti-tuberculosis agent rifampicin (RFP-PLGA MS) has been shown to be effective for the treatment of tuberculosis. The phagocytosis should be evaluated in terms of that toward reference MS. We chose polystyrene latex (PSQ MS as a reference. In this study, phagocytic activity of cell line NR8383, derived from rat alveolar M phi, toward PSL MS with various diameters was examined by incubating the cells for 4 h at 37 degrees C with various numbers of PSL MS per M phi cell (MS/M phi = 0.1-10). The results were then compared with those of the phagocytosis toward RFP-PLGA MS. We determined the phagocytic activity by counting the population of M phi cells that had phagocytosed MS (N) and the number of particles phagocytosed (n) in microscopic fields. Both N and n for PSL and RFP-PLGA MS increased in general with an increase in MS/M phi but both of these values for PSL MS were smaller than those for RFP-PLGA MS. Phagocytosis of the particles were dependent on the particle size; i.e., of the PSL MS the 6-mu m ones were taken up by M phi the most, and the RFP-PLGA MS 3 mu m in diameter seemed to be phagocytosed the most efficiently, although we were not able to determine exactly the phagocytosis of 6- and 10-mu m RFP-PLGA MS. From the changes in N and n values with MS/M phi, the phagocytosis of RFP-PLGA MS was likely to enhance the phagocytic activity of M phi cells, but this effect did not seem to be significant for PSL MS. (C) 2007 Elsevier B.V. All rights reserved.