A Comparative Study on the Incidence, Aggravation, and Remission of Lupus Nephritis Based on iTRAQ Technology | Bentham Science

摘要

Aim and Objective: Lupus nephritis (LN) is one of the major complications of systemiclupus erythematosus (SLE). The specific mechanisms of pathogenesis, aggravation, and remissionprocesses in LN have not been clarified but is of great need in the clinic. Using isobaric tags forrelative and absolute quantitation (iTRAQ) technology to screen the functional proteins of LN inmice. Especially under intervention factors of lipopolysaccharide (LPS) and dexamethasone.Methods: Mrl-lps mice were intervened with LPS, dexamethasone, and normal saline(NS) usingintraperitoneal injection, and c57 mice intervened with NS as control. The anti-ANA antibodyenzyme-linked immunosorbent assay (ELISA) was used to verify disease severity. Kidney tissue iscollected and processed for iTRAQ to screen out functional proteins closely related to the onsetand development of LN. Western blot method and rt-PCR (real-time Polymerase Chain Reaction)were used for verification.Results: We identified 136 proteins that marked quantitative information. Among them, Hp,Igkv8-27, Itgb2, Got2, and Pcx proteins showed significant abnormal manifestations.Conclusion: Using iTRAQ methods, the functional proteins Hp, Igkv8-27, Itgb2, Got2, and Pcxwere screened out for a close relationship with the pathogenesis and development of LN, which isworth further study.