Production of taxol from in vitro cell culture of Taxus cuspidata

摘要

Taxol, a diterpenoid extracted from Taxus spp., is used to treat ovarian cancer. Methods of increasing taxol production by cell cultures of T. cuspidata, derived from stem segments, were investigated. Increasing the ratio of nitrate:ammonium from 1:1 to 30:1 increased cell growth but had an adverse effect on taxol content. Nitrate alone was a better source of N than ammonium alone. Reducing the N or P concentration slowed cell growth but improved taxol production. Immobilizing the cells on alginatebeads also increased taxol production.