Serological detection of strawberry mild yellow edge-associated virus

摘要

Monoclonal and polyclonal antibodies were produced against the potexvirus associated with strawberry mild yellow edge disease. For the polyclonal antiserum strawberry mild yellow edge-associated potexvirus (SMYEAV) was partially purified from Chenopodium quinoa co-infected with humulus japonicus ilarvirus. For monoclonal antibody production, mice were immunised with preparations of SMYEAV that were partially purified from aphid-inoculated Fragaria vesca UC-5 plants. The monoclonal antibody EMA-48 was used in indirect double-antibody ELISA to trap antigen in combination with polyclonal detecting antibodies. In this way SMYEAV was detected in samples from F. vesca infected with the homologous virus isolate, 2 isolates from Germany and from 14 commercial strawberry plants from southern England. The results were similar to those obtained using an all-polyclonal ELISA system and indicated the broad reactivity of EMA-48. During the summer, SMYEAV was detected in samples from leaves, flowers and roots of commercial strawberry plants; strongest reactions were produced by samples from petals and from old leaves. In host range studies, 38 species in 15 families were inoculated with SMYEAV by sap and/or aphids without finding a satisfactory propagation host, although the virus has been maintained for more than 6 consecutive passages in inoculated leaves of G. foliosum.