Immunohistochemical Analysis of Copper Chaperone for Superoxide Dismutase in Mouse Central Nervous System; a Comparative Study with Paraffin and Frozen Section

摘要

To examine the immunohistochemical localization of copper chaperone for superoxide dismutase (CCS) in the mouse central nervous system (CNS), we produced an affinity purified polyclonal antibody against mouse CCS and compared paraffin sections with non-fixed or fixed frozen sections. Pyramidal neurons, Purkinje cells and anterior horn cells were intensely stained. The immunoreactivity was also present in other neurons throughout the CNS. The staining pattern with paraffin sections was similar to that with non-fixed and fixed frozen sections, indicating that the antibody could be used for the immunohistochemical investigations including localization of CCS in the motor neurons of familial amyotrophic lateral sclerosis model mice using permanent paraffin blocks.