An alternative pathway for plant in vitro regeneration of insecticidal tree Melia azedarach L. derived from the induction of somatic embryogenesis.

摘要

A model for highly efficient method for plant regeneration from somatic embryos of chinaberry tree (M. azedarach) was obtained. Mature seeds were cultured on Murashige and Skoog's medium supplemented with NAA/BA to induce embryogenic tissues. Embryo proliferation occurred with high level cytokinin concentration (10 mg/litre BAP [benzyladenine]) and germination occurred on free hormone medium or in BAP/AG3 medium. Plants were regenerated from both, direct embryo formation and through an intermediary callus phase. Results showed 100% embryo conversion to plant. Regenerated plants were normal phenotypically and continued to grow after transfer to a greenhouse environment with 100% of surviving with high rate of growth..