Breeding of homozygotes of self-incompatible haplotype in Japanese pear (Pyrus pyrifolia Nakai).

摘要

A laboratory study was conducted to select S4, S5 and S7 homozygotes in Japanese pear. 421-6 and 421-24, selected from seedlings obtained from the selfing of Shinsui (S4S5), are compatible with the pollen of Shinsui. The pollen of 421-6 is incompatible with Yakumo (S1S4) and Gold Nijisseiki (S2S4), but compatible with Housui (S3S5). On the other hand, the pollen of 421-24 is incompatible with Housui and Okusankichi (S5S7), but compatible with Yakumo (S1S4). 420-50, which was selected from the selfed seedlings of Okusankichi, is compatible with the pollen of Okusankichi. The pollen of 420-50 is incompatible with Hougetsu (S1S7), but compatible with Housui. The PCR-RFLP method showed only one S-RNase fragment in these 3 lines; that of 421-6 is digested by NdeI, that of 421-24 is digested by AlwNI and that of 420-50 is digested by AccII. The S-genotypes of 421-6, 421-24 and 420-50 are S4S4, S5S5 and S7S7, respectively..