QM/MM Investigation for Protonation States in a Bilin Reductase PcyA‐Biliverdin IXɑ Complex

Iijima Eri; Gleeson M. Paul; Unno Masaki; Mori Seiji

摘要

Abstract > Herein we report quantum mechanical/molecular mechanical (QM/MM) studies to investigate the most probable protonation states of active site amino acids and bound substrate based on a recently reported neutron diffraction structure of phycocyanobilin:ferredoxin oxidoreductase (PcyA) by Unno et?al. This structure was considered to be bound in its initial state of biliverdin IXɑ (BV), which has the C‐pyrrole ring in the deprotonated state. The protonation state of BV suggested by neutron and spectroscopic studies is a stable, two‐electron reduced complex with a bound hydronium ion. Several ambiguities in the neutron structure were observed which prompted a further theoretical analysis of the structure. This structural investigation provides new understanding of the PcyA and BV protonation states not previously reported in the literature. Our calculations suggest that the hydronium ion (H <sub>3</sub> O + ) is energetically unfavorable, preferentially protonating the neighboring His88 residue and that the C‐ring of BV is not protonated. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>摘要</标题> >在此报告量子机械/分子机械（qm / mm）研究以研究最有可能的研究基于最近报道的植物中的中子衍射结构的激活位点氨基酸和结合衬底的质子化状态：Unno等，氧化丁蛋白氧化酶（PCya）氧化嗪氧化酶（PCya）。认为这种结构是在其初始状态的胆汁丁蛋白（BV）的初始状态，其在去质子化状态下具有C-吡咯环。通过中子和光谱研究建议的BV的质子化状态是稳定的两电子，其具有结合的纳温离子。观察到中子结构中的几个歧义，促使对结构的进一步理论分析。这种结构调查为先前报道的PCYA和BV质子化状态提供了新的了解。我们的计算表明，纳温离子（H <Sub> 3 </ sub> O + </ sup>）在能量上是不利的，优先对邻近的HIS88残基进行质子，并且BV的C形环未质子化。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-16300/'>《Chemphyschem: A European journal of chemical physics and physical chemistry》</a> <b style="margin: 0 2px;">|</b><span>2018年第15期</span><b style="margin: 0 2px;">|</b><span>共5页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Iijima Eri&option=202" target="_blank" rel="nofollow">Iijima Eri;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gleeson M. Paul&option=202" target="_blank" rel="nofollow">Gleeson M. Paul;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Unno Masaki&option=202" target="_blank" rel="nofollow">Unno Masaki;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mori Seiji&option=202" target="_blank" rel="nofollow">Mori Seiji;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Institute of Quantum Beam Science Graduate School of Science and EngineeringIbaraki UniversityMito 310–8512 and Hitachi 316–8511 Ibaraki Japan;</p> <p>Department of Biomedical Engineering Faculty of EngineeringKing Mongkut's institute of Technology LadkrabangThailand;</p> <p>Institute of Quantum Beam Science Graduate School of Science and EngineeringIbaraki UniversityMito 310–8512 and Hitachi 316–8511 Ibaraki Japan;</p> <p>Institute of Quantum Beam Science Graduate School of Science and EngineeringIbaraki UniversityMito 310–8512 and Hitachi 316–8511 Ibaraki Japan;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1186.html" title="物理化学（理论化学）、化学物理学">物理化学（理论化学）、化学物理学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=ONIOM Calculations&option=203" rel="nofollow">ONIOM Calculations;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=PcyA&option=203" rel="nofollow">PcyA;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=enzyme&option=203" rel="nofollow">enzyme;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=protonation state&option=203" rel="nofollow">protonation state;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=oxidoreductase&option=203" rel="nofollow">oxidoreductase;</a> </p> <div class="translation"> 机译：oniom计算;pcya;酶;质子化状态;氧化还原酶; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chinese-journal-chemical-engineering_thesis/0201288870794.html">Asymmetric bioreduction of γ-and δ-keto acids by native carbonyl reductases from Saccharomyces cerevisiae</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chunlei Ren&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Chunlei Ren,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Tao Wang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Tao Wang,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Xiaoyan Zhang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Xiaoyan Zhang,</a> <a href="/journal-foreign-9041/" target="_blank" rel="nofollow" class="tuijian_authcolor">中国化学工程学报（英文版） .</a> <span>2021</span><span>,第001期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-agricultural-sciences-english_thesis/02012297363.html">Responses of N2O reductase gene （nosZ）-denitrifier communities to long-term fertilization follow a depth pattern in calcareous purplish paddy soil</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=WANG Ying-yan&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">WANG Ying-yan,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=LU Sheng-e&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">LU Sheng-e,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=XIANG Quan-ju&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">XIANG Quan-ju,</a> <a href="/journal-foreign-18663/" target="_blank" rel="nofollow" class="tuijian_authcolor">农业科学学报：英文版 .</a> <span>2017</span><span>,第011期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-integrative-agriculture_thesis/020126160274.html">Responses of N2O reductase gene (nosZ)-denitrifier communities to long-term fertilization follow a depth pattern in calcareous purplish paddy soil</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=WANG Ying-yan&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">WANG Ying-yan,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=LU Sheng-e&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">LU Sheng-e,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=XIANG Quan-ju&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">XIANG Quan-ju,</a> <a href="/journal-foreign-7366/" target="_blank" rel="nofollow" class="tuijian_authcolor">农业科学学报（英文版） .</a> <span>2017</span><span>,第011期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-hainan-medical-university-english-edition_thesis/0201235043558.html">Correlation of methylenetetrahydrofolate reductase polymorphism with Hcy metabolism and inflammatory response in patients with recurrent cerebral infarction</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gai-Zhuang Liu&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Gai-Zhuang Liu </a> <a href="/journal-foreign-11892/" target="_blank" rel="nofollow" class="tuijian_authcolor">海南医科大学学报（英文版） .</a> <span>2017</span><span>,第013期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704022114584.html">QM/MM Investigation for Protonation States in a Bilin Reductase PcyA‐Biliverdin IXɑ Complex</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Iijima Eri&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Iijima Eri,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gleeson M. Paul&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Gleeson M. Paul,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Unno Masaki&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Unno Masaki,</a> <a href="/journal-foreign-16300/" target="_blank" rel="nofollow" class="tuijian_authcolor">Chemphyschem: A European journal of chemical physics and physical chemistry .</a> <span>2018</span><span>,第15期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：QM / MM对Bilin Reductase PCYA-BiliverdinIxń复合体的质子化状态调查</span> </p> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704028102214.html">Crystal Growth of a Bilin Reductase PcyA I86D Mutant-Substrate Complex for Neutron Crystallography</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Igarashi Keisuke&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Igarashi Keisuke,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hagiwara Yoshinori&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Hagiwara Yoshinori,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sugishim Masakazu&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Sugishim Masakazu,</a> <a href="/journal-foreign-17822/" target="_blank" rel="nofollow" class="tuijian_authcolor">Crystal growth & design .</a> <span>2018</span><span>,第9期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：Bilin还原酶PCYA I86D突变体 - 基质复合物用于中子晶体学的晶体生长</span> </p> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704028102117.html">Crystal Growth of a Bilin Reductase PcyA I86D Mutant-Substrate Complex for Neutron Crystallography</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Igarashi Keisuke&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Igarashi Keisuke,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hagiwara Yoshinori&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Hagiwara Yoshinori,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sugishim Masakazu&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Sugishim Masakazu,</a> <a href="/journal-foreign-17822/" target="_blank" rel="nofollow" class="tuijian_authcolor">Crystal growth & design .</a> <span>2018</span><span>,第9期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：Bilin还原酶PCYA I86D突变体 - 基质复合物用于中子晶体学的晶体生长</span> </p> </li> <li> <div> <b>8. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_international-conference-computational-methods-sciences-engineering_thesis/020512234667.html">Simulation of Proton Transfer Processes and Excited-State Properties in Proteins with QM/MM Methods</a> <b>[C]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=M. Elstner&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">M. Elstner,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=M. Hoffmann&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">M. Hoffmann,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=M. Wanko&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">M. Wanko </a> <a href="/conference-foreign-34125/" target="_blank" rel="nofollow" class="tuijian_authcolor">International Conference of Computational Methods in Sciences and Engineering 2007(ICCMSE 2007); 20070925-30; Corfu(GR) .</a> <span>2007</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：QM / MM方法模拟蛋白质的质子转移过程和激发态性质</span> </p> </li> <li> <div> <b>9. </b><a class="enjiyixqcontent" href="/academic-degree-foreign_mphd_thesis/02061201055.html">Structure, Function, and Dynamics of Mutant PcyA, the Archetypal Member of the Ferredoxin-Dependent Bilin Reductase Family.</a> <b>[D] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gae, David Donghyon.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Gae, David Donghyon. </a> <span>2013</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：结构，功能和动力学突变PcyA，铁氧还蛋白依赖性Bilin还原酶家族的原型成员。</span> </p> </li> <li> <div> <b>10. </b><a class="enjiyixqcontent" href="/academic-journal-foreign-pmc_detail_thesis/040005254792.html">Characterization of Ferredoxin-Dependent Biliverdin Reductase PCYA1 Reveals the Dual Function in Retrograde Bilin Biosynthesis and Interaction With Light-Dependent Protochlorophyllide Oxidoreductase LPOR in Chlamydomonas reinhardtii</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Weiqing Zhang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Weiqing Zhang,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Huan Zhong&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Huan Zhong,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hui Lu&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Hui Lu,</a> <span>-1</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：表征铁氧还蛋白依赖的Biliverdin还原酶PCYA1揭示了逆境Bilin生物合成中的双重功能以及与莱茵衣藻中光依赖性原叶绿素氧化物氧化还原酶LPOR的相互作用。</span> </p> </li> <li> <div> <b>11. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/01000126473881.html">The use of synthetic linear tetrapyrroles to probe the verdin sites of human biliverdin-IXα reductase and human biliverdin-IXβ reductase</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Edward M. Franklin&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Edward M. Franklin,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Seamus Browne&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Seamus Browne,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Anne M. Horan&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Anne M. Horan,</a> <span>2009</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：使用合成线性四吡咯来探测人Biliverdin-IXα还原酶和人Biliverdin-Ixβ还原酶的Verdin位点</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_acta-physico-chimica-sinica_thesis/0201251022133.html">Quantifying Electronic Effects in QM and QM/MM Biomolecular Modeling with the Fukui Function</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=QI Helena W.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . QI Helena W.</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=KARELINA Maria&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,KARELINA Maria</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=KULIK Heather J.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,KULIK Heather J.</a> <span> <a href="/journal-cn-15102/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 物理化学学报 </a> </span> <span> . 2018</span><span>,第001期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-10_thesis/02022738.html">基于QM/MM模型的外二元醇双加氧酶催化机理研究</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘洋&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 刘洋</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=涂宁宇&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,涂宁宇</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=谢文玉&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,谢文玉</a> <span> <a href="/conference-cn-10/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 2017中国环境科学学会科学与技术年会 </a> <span> <span> . 2017</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020313311844.html">TCR-peptidE-MHC I类蛋白质复合体相互作用QM/MM分子动力学模拟研究</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=隋文鹏&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 隋文鹏</a> <span> . 2015</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/061203480797.html">生命体系中等位基因竞争性反应QM/MM方法</a> <b>[P]</b> . <span> 中国专利： CN106355025B </span> <span> . 2019.09.20</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120108832970.html">生命体系中等位基因竞争性反应QM/MM方法</a> <b>[P]</b> . <span> 中国专利： CN106355025A </span> <span> . 2017-01-25</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130401782911.html">Methods for increasing platelet count by inhibiting biliverdin IXβ reductase</a> <b>[P]</b> . <span> 外国专利：  US10543286B2 </span> <span> . 2020-01-28</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：通过抑制biliverdinIXβ还原酶增加血小板计数的方法 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130408938407.html">METHODS FOR INCREASING PLATELET COUNT BY INHIBITING BILIVERDIN IXβ REDUCTASE</a> <b>[P]</b> . <span> 外国专利：  WO2017062422A1 </span> <span> . 2017-04-13</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：抑制生物素IXβ还原酶增加血小板计数的方法 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130414218296.html">THERAPEUTIC AND PHARMACEUTICAL COMPOSITION FOR SKIN INFLAMMATION CONTAINING BILIVERDIN REDUCTASE A FUSION PROTEIN</a> <b>[P]</b> . <span> 外国专利：  KR20150055246A </span> <span> . 2015-05-21</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：包含生物素还原酶和融合蛋白的皮肤炎症的治疗和药物成分 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704022114584','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="关闭" onclick="$('.journalsub-pop-up').hide()"> <p class="pardon">抱歉，该期刊暂不可订阅，敬请期待！</p> <p class="current">目前支持订阅全部北京大学中文核心（2020）期刊目录。</p> <div style="display: flex;margin-top: 69px;justify-content: space-between;"> <div class="no-sub" onclick="$('.journalsub-pop-up').hide()">暂不订阅</div> <div class="other-sub" onclick="continueSub('from=pc-detail')">继续订阅其他期刊</div> </div> </div> </div> <div class="right_btn"> <ul> <li class="gouwuche">  </li> <li class="yijian"> <a href="javascript:void(0);" onclick="link_analysis('/mycenter/auth/complaint.html',this)" title="意见反馈">意见反馈</a> </li> <li class="top"> <a href="javascript:scrollTo(0,0);" title="回到顶部">回到顶部</a> </li> <li class="shouye"> <a href="/" title="首页">回到首页</a> </li> </ul> </div> <div class="xllindexfooter"> <div class="xllindexfootercenter"> <div class="xllindexfooterleft left" > <div class="xllindexfooterleftli"> <ul> <li><a href="/about.html" title="关于掌桥">关于掌桥</a></li> <li><a href="/help/helpmap.html" title="资源导航">资源导航</a></li> <li><a href="/help/helpguide.html" title="新手指南">新手指南</a></li> <li><a href="/help/helpcenter.html" title="常见问题">常见问题</a></li> <li><a href="/sitemap.html" title="网站地图">网站地图</a></li> <li><a href="/help/helpcenter.html?type=9" title="版权声明">版权声明</a></li> </ul> </div> <div class="xllindexfooterleft"> <p class="xllindexfooterlefteamil">客服邮箱：kefu@zhangqiaokeyan.com</p> <div class="xllindexfooterlefttcp"> <div class="xllindexfooterpoliceiimg"></div> <div class="xllindexfooterpoliceispan"> <span>京公网安备：11010802029741号 </span> <span>ICP备案号：<a href="https://beian.miit.gov.cn" rel="nofollow" target="_blank" title="ICP备案号">京ICP备15016152号-6</a></span> <span>六维联合信息科技 (北京) 有限公司©版权所有</span> </div> </div> </div> </div> <div class="xllindexfooterright left"> <ul> <li> <p style="font-weight: bold;">客服微信</p> <div></div> </li> <li> <p style="font-weight: bold;">服务号</p> <div></div> </li> </ul> </div> </div> </div> <span id="0704022114584down" data-source="7," data-out-id="eBXZBEm95Z+Dz7WDlNigukoeAglkpYE+TyrvPyUNChPlwq+OAJ2KHFG6zrEd0d80," data-f-source-id="7" data-title="QM/MM Investigation for Protonation States in a Bilin Reductase PcyA‐Biliverdin IXɑ Complex" data-price="20" data-site-name="" data-transnum="24" style="display:none;"></span> <input type="hidden" value="4" id="sourcetype"> <input type="hidden" value="16300" id="journalid"> <input type="hidden" value="1" id="inyn_provide_service_level"> <input type="hidden" value="https://cdn.zhangqiaokeyan.com" id="imgcdn"> <input type="hidden" value="1" id="isdeatail"> <input type="hidden" value="" id="syyn_indexed_database"> <input type="hidden" value="" id="servicetype"> <input type="hidden" id="pagename" value="QM/MM Investigation for Protonation States in a Bilin Reductase PcyA‐Biliverdin IXɑ Complex"/> <input type="hidden" value="thesis_get_original" id="pageIdentification"> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery-1.12.4.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/lwlh_ajax.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zq.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/common.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery.cookie.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/top.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/tip.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/login.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/down.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/search.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/newmail.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/searchtype.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/user/regist.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zxs_solor/detail.js?v=5.7.8"></script> <script type="text/javascript" src="https://www.zhangqiaokeyan.com/statistics/static/pagecollection.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/tj.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/sse.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/pushbaidu.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/history.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/util/cookie.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/weipu/weipu.js?v=5.7.8"></script> </body> <script> function kefu(customization) { var from = GetQueryString("from"); if (from == null || from == "") { from = "other"; } var url = getWxWorkQrCode(55, from, customization); if (url == "" || typeof url == 'undefined') { url = "https://wework.qpic.cn/wwpic/994838_0LUTS-VqR0quT1N_1675665967/0"; } tipDocGuidance(url, '人工辅助获取外文期刊材料'); } $(function(){ var weiPuStatus = getCookie('WeiPuStatus'); if(weiPuStatus){ tipWeiPuStatus(weiPuStatus); delCookie('WeiPuStatus'); } getFacetKeywordVoInId(); var sourcetype = $("#sourcetype").val(); var inyn_provide_service_level = $("#inyn_provide_service_level").val(); if((sourcetype ==1||sourcetype==4)&&inyn_provide_service_level!=4){ getJournal(); } }) </script> </html>