Albatrellus ovinus contains a α- l-fuco-α- d-galactan, α- d-glucan, a branched (1?→?6)-β- d-glucan and a branched (1?→?3)-β- d-glucan]]>

摘要

Albatrellus ovinus, the sheep polypore, is a large, dense mushroom being rich in cell wall material. Polysaccharides were isolated by sequential extraction, enzymatic treatment and analyzed with respect to monosaccharide composition, glycosidic linkages by methylation and GC-MS as well as NMR spectroscopy. A fucogalactan composed of an (1?→?6)-α-d-galactan backbone with single α-l-Fucpresidues attached atO-2 was identified in the hot water extract obtained after treatment with a protease and size exclusion chromatography. Both the hot water extract and the hot alkali extract contained an (1?→?4)-α-d-glucan whereas β-d-glucans were mainly present in the latter. Structural analysis suggested the presence of two different β-d-glucan backbone structures; a (1?→?6)-linked β-d-glucan with single β-d-Glcpresidues atO-3 and also a (1?→?3)-linked β-d-glucan with branches in O-6. In addition there were identified short (1?→?2)-linked β-d-xylan and (1?→?3)-α-d-mannan chains.