Surface and Solvent Dependent Adsorption of Three Neuromedin-Like Peptides in Glass and Plastic Syringes

摘要

Abstract In the last decades, there is an increasing interest in the use of biomolecules, such as peptides, as therapeutic agents. One important challenge inherent to peptides and proteins is the occurrence of aspecific adsorption to all kind of surfaces, hampering drug research and development. In recent years, a lot of research was already performed highlighting adsorption during dissolution, sample pretreatment and analysis. However, only limited data is available about the loss of peptide caused by adsorption to the in vivo administration setup. For this communication we assessed the adsorption of three neuropeptides in glass and plastic syringes by measuring the neuropeptide concentration with nanoUHPLC–MS/MS as a function of time, delivered by the syringe at a flow rate of 2 μL/min. To create a more fundamental approach, three different infusion solvents were examined. Our results clearly show that—although peptide dependent—an important influence of the kind of syringe material and perfusion solvent can be seen on peptide recovery. In addition, we discuss different strategies, and their corresponding in vivo difficulties, to increase peptide recovery during administration.