Quality Control of Xiebai San Standard Decoction Assisted by Network Pharmacology Strategy

摘要

Xiebai San (XBS) is a classical herbal prescription which has been used for the treatment of infantile cough and asthma for several hundred years. In this study, 39 compounds in XBS standard decoction were identified by HPLC-ESI-TOF system. Network pharmacology strategy was adopted to explore the relationship of chemicals, target genes and pathways, mulberroside A, kukoamine B and glycyrrhizic acid were chosen as quality markers in XBS standard decoction. An ultra-high-performance liquid chromatography (UHPLC) method was established to simultaneously determine these three compounds on the Waters CORTECS T3 column (150 mm x 3 mm, 1.6 mu m) at 0.45 mL min(-1) with 0.1% (v/v) TFA (A) and acetonitrile (B) as the mobile phase. The developed method was validated with satisfactory parameters: specificity, linearity, precision, accuracy, stability and ruggedness. The method was then applied to analyze ten batches of XBS standard decoction and the initial quality control standard of XBS standard decoction was established. Graphic