A Bioorganic Chemistry Approach to Understanding Molecular Recognition in Protein-Nucleic Acid Complexes

摘要

A combination of synthetic, organic, and biochemical approaches has been developed to study how protein and nucleic acid assemblies modulate the affinity, specificity and cooperativity of protein-nucleic acid interactions. These strategies have enabled formation of noncovalent peptide dimers on specific DNA sequences. A new framework from RNA and peptide into a stable complex of ribonucleopeptide was also developed to construct receptors and fluorescent sensors for small molecules. Attempts to design novel DNA-binding peptides, receptors and sensors will provide an ultimate test for our understanding of the principle of molecular recognition associated with protein-nucleic acid interactions.