首页> 外文期刊>Acta histochemica et cytochemica >Prenatal and lactational exposure to bisphenol a in mice alters expression of genes involved in cortical barrel development without morphological changes
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Prenatal and lactational exposure to bisphenol a in mice alters expression of genes involved in cortical barrel development without morphological changes

机译:小鼠双酚a的产前和哺乳期暴露可改变皮层桶发育相关基因的表达,而无形态变化

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It has been reported that premature infants in neonatal intensive care units are exposed to a high rate of bisphenol A (BPA), an endocrine disrupting chemical. Our previous studies demonstrated that corticothalamic projection was disrupted by prenatal exposure to BPA, which persisted even in adult mice. We therefore analyzed whether prenatal and lactational exposure to low doses of BPA affected the formation of the cortical barrel, the barreloid of the thalamus, and the barrelette of the brainstem in terms of the histology and the expression of genes involved in the barrel development. Pregnant mice were injected sub-cutaneously with 20 μg/kg of BPA daily from embryonic day 0 (E0) to postnatal 3 weeks (P3W), while the control mice received a vehicle alone. The barrel, barreloid and barrelette of the adult mice were examined by cytochrome C oxidase (COX) staining. There were no significant differences in the total and septal areas and the patterning of the posterior medial barrel subfield (PMBSF), barreloid and barrelette, between the BPA-exposure and control groups in the adult mice. The developmental study at postnatal day 1 (PD1), PD4 and PD8 revealed that the cortical barrel vaguely appeared at PD4 and completely formed at PD8 in both groups. The expression pattern of some genes was spatiotemporally altered depending on the sex and the treatment. These results suggest that the trigeminal projection and the thalamic relay to the cortical barrel were spared after prenatal and lactational exposure to low doses of BPA, although prenatal exposure to BPA was previously shown to disrupt the corticothalamic projection.
机译:据报道,新生儿重症监护室的早产儿暴露于高比率的双酚A(BPA),这是一种破坏内分泌的化学物质。我们之前的研究表明,产前暴露于双酚A会破坏皮层丘脑投射,甚至在成年小鼠中也会持续存在。因此,我们从组织学和参与桶发展的基因表达的角度分析了产前和哺乳期低剂量BPA暴露是否影响皮质桶,丘脑的桶状体和脑干的桶状体的形成。从胚胎第0天(E0)到出生后3周(P3W),每天对皮下注射妊娠小鼠皮下注射20μg/ kg的BPA,而对照组则仅接受媒介物。通过细胞色素C氧化酶(COX)染色检查成年小鼠的桶状,桶状和桶状。在成年小鼠的BPA暴露组和对照组之间,总面积和间隔面积以及后内侧桶形子区域(PMBSF),桶形和桶形的图案均无显着差异。出生后第1天(PD1),PD4和PD8的发育研究表明,两组的皮质桶模糊地出现在PD4,并在PD8完全形成。某些基因的表达方式会根据性别和治疗方式在时空上发生改变。这些结果表明,尽管在出生前和哺乳期暴露于低剂量的BPA后,三叉神经投射和丘脑对皮层桶的中继仍可幸免,尽管以前显示对BPA的产前暴露会破坏皮层丘脑投射。

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