A Photoenzymatic NADH Regeneration System

H?fler Georg T.; Fernández‐Fueyo Elena; Pesic Milja; Younes Sabry H.; Choi Eun‐Gyu; Kim Yong H.; Urlacher Vlada B.; Arends Isabel W. C. E.; Hollmann Frank

摘要

Abstract > A photoenzymatic NADH regeneration system was established. The combination of deazariboflavin as a photocatalyst with putidaredoxin reductase enabled the selective reduction of NAD + into the enzyme‐active 1,4‐NADH to promote an alcohol dehydrogenase catalysed stereospecific reduction reaction. The catalytic turnover of all the reaction components was demonstrated. Factors influencing the efficiency of the overall system were identified. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> >一个光酶NADH再生系统。 Deazariboflavin作为光催化剂的结合，具有普发脱毒素还原酶，使NAD <SUP> + </ SOP>的选择性还原为酶 - 活性1,4-NADH，以促进醇脱氢酶催化的立体特异性还原反应。证明了所有反应组分的催化转余。确定了影响整体系统效率的因素。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-16039/'>《Chembiochem: A European journal of chemical biology》</a> <b style="margin: 0 2px;">|</b><span>2018年第22期</span><b style="margin: 0 2px;">|</b><span>共4页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=H?fler Georg T.&option=202" target="_blank" rel="nofollow">H?fler Georg T.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fernández‐Fueyo Elena&option=202" target="_blank" rel="nofollow">Fernández‐Fueyo Elena;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Pesic Milja&option=202" target="_blank" rel="nofollow">Pesic Milja;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Younes Sabry H.&option=202" target="_blank" rel="nofollow">Younes Sabry H.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Choi Eun‐Gyu&option=202" target="_blank" rel="nofollow">Choi Eun‐Gyu;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kim Yong H.&option=202" target="_blank" rel="nofollow">Kim Yong H.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Urlacher Vlada B.&option=202" target="_blank" rel="nofollow">Urlacher Vlada B.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Arends Isabel W. C. E.&option=202" target="_blank" rel="nofollow">Arends Isabel W. C. E.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hollmann Frank&option=202" target="_blank" rel="nofollow">Hollmann Frank;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of BiotechnologyDelft University of Technologyvan der Maasweg 9 2629 HZ Delft The Netherlands;</p> <p>Department of BiotechnologyDelft University of Technologyvan der Maasweg 9 2629 HZ Delft The Netherlands;</p> <p>Department of BiotechnologyDelft University of Technologyvan der Maasweg 9 2629 HZ Delft The Netherlands;</p> <p>Department of BiotechnologyDelft University of Technologyvan der Maasweg 9 2629 HZ Delft The Netherlands;</p> <p>School of Energy and Chemical EngineeringUlsan National Institute of Science and TechnologyUlsan 689–798 South Korea;</p> <p>School of Energy and Chemical EngineeringUlsan National Institute of Science and TechnologyUlsan 689–798 South Korea;</p> <p>Chair of Biochemistry IIHeinrich Heine University DüsseldorfUniversit?tsstra?e 1 40225 Düsseldorf Germany;</p> <p>Department of BiotechnologyDelft University of Technologyvan der Maasweg 9 2629 HZ Delft The Netherlands;</p> <p>Department of BiotechnologyDelft University of Technologyvan der Maasweg 9 2629 HZ Delft The Netherlands;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/179.html" title="分子生物学">分子生物学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=biocatalysis&option=203" rel="nofollow">biocatalysis;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=enantioselectivity&option=203" rel="nofollow">enantioselectivity;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=ketones&option=203" rel="nofollow">ketones;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=photochemistry&option=203" rel="nofollow">photochemistry;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=reduction&option=203" rel="nofollow">reduction;</a> </p> <div class="translation"> 机译：生物分析;对映选择性;酮;光化学;减少; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div 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href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=杨光&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,杨光</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=裴海生&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,裴海生</a> <span> <a href="/journal-cn-56742/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 过程工程学报 </a> </span> <span> . 2008</span><span>,第2期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_detail_thesis/0201293234963.html">在Klebsiella pneumoniae醛脱氢酶失活菌中构建NADH再生系统</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=黄志华&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 黄志华</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张延平&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,张延平</a> <a 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tuijian_authcolor">,张崇富</a> <span> <a href="/journal-cn-4594/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 电子科技大学学报 </a> </span> <span> . 2004</span><span>,第006期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-xidian-university_thesis/0201211054469.html">一种新型的光折变自适应光外差探测系统</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=许海平&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 许海平</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=石顺祥&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,石顺祥</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=孙艳玲&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,孙艳玲</a> <span> <a href="/journal-cn-7628/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 西安电子科技大学学报（自然科学版） </a> </span> <span> . 2002</span><span>,第005期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-cn_designing-techniques-posts-telecommunications_thesis/0201297195143.html">监测全塑电（光）缆线路系统的一种新方法──NORSCAN—2000型电（光）</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈昭桐&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 陈昭桐</a> <span> <a href="/journal-cn-8800/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 邮电设计技术 </a> </span> <span> . 1995</span><span>,第3期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-24896_thesis/020221258216.html">暗室和光助Co2+/KHSO5系统对AO7的脱色和矿化动力学研究</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈晓旸&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 陈晓旸</a> <a 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