Rational Design of Domain‐Swapping‐Based        <i >cc        ‐Type Cytochrome Heterodimers by Using Chimeric Proteins

Zhang Mohan; Nakanishi Tsukasa; Yamanaka Masaru; Nagao Satoshi; Yanagisawa Sachiko; Shomura Yasuhito; Shibata Naoki; Ogura Takashi; Higuchi Yoshiki; Hirota Shun

摘要

Abstract > The design of protein oligomers with multiple active sites has been gaining interest, owing to their potential use for biomaterials, which has encouraged researchers to develop a new design method. Three‐dimensional domain swapping is the unique phenomenon in which protein molecules exchange the same structural region between each other. Herein, to construct oligomeric heme proteins with different active sites by utilizing domain swapping, two c ‐type cytochrome‐based chimeric proteins have been constructed and the domains swapped. According to X‐ray crystallographic analysis, the two chimeric proteins formed a domain‐swapped dimer with two His/Met coordinated hemes. By mutating the heme coordination structure of one of the two chimeric proteins, a domainswapped heterodimer with His/Met and His/H <sub>2</sub> O coordinated hemes was formed. Binding of an oxygen molecule to the His/H <sub>2</sub> O site of the heterodimer was confirmed by resonance Raman spectroscopy, in which the Fe?O <sub>2</sub> stretching band was observed at 580?cm ?1 for the reduced/oxygenated heterodimer (at 554?cm ?1 under an 18 O <sub>2</sub> atmosphere). These results show that domain swapping is a useful method to design multiheme proteins. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“main”XML：Lang =“en”> <标题类型=“main”>摘要</ title> >由于其潜在使用，多个活跃点的蛋白质低聚物的设计对于生物材料，鼓励研究人员开发一种新的设计方法。三维域交换是蛋白质分子在彼此之间交换相同的结构区域的独特现象。在此，通过利用结构域交换构建具有不同活性位点的低聚血红素蛋白，已经构建了两种 C </ i>型基于细胞色素的嵌合蛋白，并且圆锥形结构域。根据X射线结晶分析，两种嵌合蛋白形成畴 - 旋转二聚体，其两个/满足其配位溶质。通过突变两个嵌合蛋白之一的血红素配位结构，形成与他/满足的多聚二二聚体，并形成His / h <sub> 2℃配位亚粒。通过共振拉曼光谱证实了氧分子与His / h <sub> 2 </ sub> O位点的结合，其中在580处观察到Fe othα0<sub> 2 </ sub>拉伸带用于氧化/氧化异二聚体的Cm β1</ sup>（在 18 / sup> o <sub> 2 2 15℃下）大气层）。这些结果表明，域交换是设计多血液蛋白的有用方法。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-16039/'>《Chembiochem: A European journal of chemical biology》</a> <b style="margin: 0 2px;">|</b><span>2017年第17期</span><b style="margin: 0 2px;">|</b><span>共4页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Mohan&option=202" target="_blank" rel="nofollow">Zhang Mohan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nakanishi Tsukasa&option=202" target="_blank" rel="nofollow">Nakanishi Tsukasa;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yamanaka Masaru&option=202" target="_blank" rel="nofollow">Yamanaka Masaru;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nagao Satoshi&option=202" target="_blank" rel="nofollow">Nagao Satoshi;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yanagisawa Sachiko&option=202" target="_blank" rel="nofollow">Yanagisawa Sachiko;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Shomura Yasuhito&option=202" target="_blank" rel="nofollow">Shomura Yasuhito;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Shibata Naoki&option=202" target="_blank" rel="nofollow">Shibata Naoki;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ogura Takashi&option=202" target="_blank" rel="nofollow">Ogura Takashi;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Higuchi Yoshiki&option=202" target="_blank" rel="nofollow">Higuchi Yoshiki;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hirota Shun&option=202" target="_blank" rel="nofollow">Hirota Shun;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Graduate School of Materials ScienceNara Institute of Science and Technology8916-5 Takayama Ikoma Nara 630-0192 Japan;</p> <p>Graduate School of Materials ScienceNara Institute of Science and Technology8916-5 Takayama Ikoma Nara 630-0192 Japan;</p> <p>Graduate School of Materials ScienceNara Institute of Science and Technology8916-5 Takayama Ikoma Nara 630-0192 Japan;</p> <p>Graduate School of Materials ScienceNara Institute of Science and Technology8916-5 Takayama Ikoma Nara 630-0192 Japan;</p> <p>Graduate School of Life ScienceUniversity of Hyogo1-1-1 Koto Sayo-cho Sayo-gun Hyogo 679-5148 Japan;</p> <p>Graduate School of Science and EngineeringIbaraki University4-12-1 Nakanarusawa Hitachi Ibaraki 316-8511 Japan;</p> <p>Graduate School of Life ScienceUniversity of Hyogo3-2-1 Koto Kamigori-cho Ako-gun Hyogo 678-1297 Japan;</p> <p>Graduate School of Life ScienceUniversity of Hyogo1-1-1 Koto Sayo-cho Sayo-gun Hyogo 679-5148 Japan;</p> <p>Graduate School of Life ScienceUniversity of Hyogo3-2-1 Koto Kamigori-cho Ako-gun Hyogo 678-1297 Japan;</p> <p>Graduate School of Materials ScienceNara Institute of Science and Technology8916-5 Takayama Ikoma Nara 630-0192 Japan;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/179.html" title="分子生物学">分子生物学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=cytochromes&option=203" rel="nofollow">cytochromes;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=domain swapping&option=203" rel="nofollow">domain swapping;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=heme proteins&option=203" rel="nofollow">heme proteins;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=oligomers&option=203" rel="nofollow">oligomers;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=protein design&option=203" rel="nofollow">protein design;</a> </p> <div class="translation"> 机译：细胞变色;域交换;血红素蛋白;低聚物;蛋白质设计; </div> </li> </ul> </div> </div> <div class="literature cardcommon" id="literaturereference" style="display:none"> <div class="similarity "> <h3 class="all_title" id="enpatent111">引文网络</h3> <div class="referencetab clearfix"> <ul id="referencedaohang"> <li dataid="referenceul">参考文献</li> <li dataid="citationul">引证文献</li> <li dataid="commonreferenceul">共引文献</li> <li dataid="commoncitationul">同被引文献</li> <li dataid="tworeferenceul">二级参考文献</li> <li dataid="twocitationul">二级引证文献</li> </ul> </div> <div class="reference_details" id="referenceList"> <ul id="referenceul"></ul> <ul id="citationul"></ul> <ul id="commonreferenceul"></ul> <ul id="commoncitationul"></ul> <ul id="tworeferenceul"></ul> <ul id="twocitationul"></ul> </div> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704022056149.html">Rational Design of Domain‐Swapping‐Based <i >cc ‐Type Cytochrome Heterodimers by Using Chimeric Proteins</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Mohan&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Zhang Mohan,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nakanishi Tsukasa&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Nakanishi Tsukasa,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yamanaka Masaru&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Yamanaka Masaru,</a> <a href="/journal-foreign-16039/" target="_blank" rel="nofollow" class="tuijian_authcolor">Chembiochem: A European journal of chemical biology .</a> <span>2017</span><span>,第17期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于结构域交换的型 C </ i> C型细胞色素异二聚体的合理设计通过使用嵌合蛋白</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-foreign_proceedings-national-academy-sciences-united-states-america_thesis/020416948905.html">Structure-based approach to rationally design a chimeric protein for an effective vaccine against Group B Streptococcus infections</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Annalisa Nuccitelli&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Annalisa Nuccitelli,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Roberta Cozzi&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Roberta Cozzi,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Louise J. Gourlay&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Louise J. Gourlay,</a> <a href="/journal-foreign-6544/" target="_blank" rel="nofollow" class="tuijian_authcolor">Proceedings of the National Academy of Sciences of the United States of America .</a> <span>2011</span><span>,第25期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于结构的方法合理设计嵌合蛋白，用于抗B组链球菌感染的有效疫苗</span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/070407846420.html">Helix swapping leads to dimerization of the N-terminal domain of the c-type cytochrome maturation protein CcmH from Escherichia coli.</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ahuja U&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Ahuja U,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Rozhkova A&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Rozhkova A,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Glockshuber R&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Glockshuber R,</a> <a href="/journal-foreign-26239/" target="_blank" rel="nofollow" class="tuijian_authcolor">FEBS letters. .</a> <span>2008</span><span>,第18期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：螺旋交换导致大肠杆菌的c型细胞色素成熟蛋白CcmH的N端结构域二聚化。</span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_meeting-191036_thesis/020512929678.html">The use of insertion sequences to analyse gene function in thiobacilus ferrooxidans: a case study involving cytochrome c-type biogenesis proteins in iron oxidation</a> <b>[C]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=D.S.Holmes&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">D.S.Holmes,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=E.Jedlicki&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">E.Jedlicki,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=M.E.Cabrejos&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">M.E.Cabrejos,</a> <a href="/conference-foreign-191036/" target="_blank" rel="nofollow" class="tuijian_authcolor">International biohydrometallurgy symposium;IBS'99 .</a> <span>1999</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：利用插入序列分析氧化硫硫杆菌的基因功能：一个涉及铁氧化中细胞色素c型生物合成蛋白的案例研究</span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-degree-foreign_mphd_thesis/02061773462.html">Evaluating the Interaction between the Human Mealanocortin-2 Receptor and the Accessory Protein, Mrap1: Chimeric Receptor and Alanine Substitution Studies on Transmembrane Domain 4, Extracellular Loop 2, and Transmembrane Domain 5</a> <b>[D] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Davis, Perry&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Davis, Perry </a> <span>2018</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：评价人类Mealanocortin-2受体与辅助蛋白Mrap1：嵌合受体和丙氨酸替代研究跨膜域4，细胞外环2和跨膜域5之间的相互作用。</span> </p> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-journal-foreign-pmc_detail_thesis/040002286246.html">From the Cover: Structure-based approach to rationally design a chimeric protein for an effective vaccine against Group B Streptococcus infections</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Annalisa Nuccitelli&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Annalisa Nuccitelli,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Roberta Cozzi&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Roberta Cozzi,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Louise J. 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Gourlay,</a> <span>2011</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：从封面开始：基于结构的方法合理设计嵌合蛋白可用于抗B组链球菌感染的有效疫苗</span> </p> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/0100013249010.html ">Helix swapping leads to dimerization of the N-terminal domain of the c-type cytochrome maturation protein CcmH from Escherichia coli</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ahuja, U.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Ahuja, U.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Rozhkova, A.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Rozhkova, A.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Glockshuber, R.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Glockshuber, R.,</a> <span>2008</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：螺旋交换导致大肠杆菌的c型细胞色素成熟蛋白CcmH的N端结构域二聚化</span> </p> </li> <li> <div> <b>8. </b><a class="enjiyixqcontent" href="/ntis-science-report_ad_thesis/020711202523.html">Evolution and Analysis of the Functional Domains of the Chimeric Proteins That Initiate Pyrimidine Biosynthesis.</a> <b>[R] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Evans, D. 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Rational Design of Domain‐Swapping‐Based cc ‐Type Cytochrome Heterodimers by Using Chimeric Proteins

摘要