Genome Mining of the Lipopeptide Biosynthesis of        <i >Paenibacillus polymyxaPaenibacillus polymyxa        E681 in Combination with Mass Spectrometry: Discovery of the Lipoheptapeptide Paenilipoheptin

Vater Joachim; Herfort Stefanie; Doellinger Joerg; Weydmann Max; Borriss Rainer; Lasch Peter

摘要

Abstract > Paenibacillus polymyxa strains are qualified for agro‐biotechnological uses such as plant growth promotion and for biocontrol strategies against deleterious phytopathogenic competitors in the soil depending on their attractive arsenal of bioactive compounds. Moreover, they are potent producers of antibiotics for medical applications. To identify new products of such organisms, genome mining strategies in combination with mass spectrometry are the methods of choice. Herein, we performed such studies with the Paenibacillus strain?E681. Bioinformatic evaluation of its genome sequence revealed four gene clusters A–D encoding nonribosomal peptide synthetases (NRPSs). Accordingly, four lipopeptide families were detected by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS). Clusters?A and D codify the well known fusaricidins and polymyxins. A yet‐unknown lipoheptapeptide was discovered and structurally characterized by de?novo sequencing by using MALDI‐LIFT‐TOF/TOF MS. It was designated as paenilipoheptin. From structure predictions we infer that the production of this agent is encoded by gene cluster?C. Gene cluster?B encodes the synthesis of tridecaptins, a family of open‐chain lipotridecapeptides. Strain E681 produces new subspecies of such compounds (tridecaptins?E) showing variations both in their fatty‐acid part as well as in their peptide part. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > Paenibacillus polymyxa </ i>菌株有资格获得农业生物技术的用途植物增长促进和对土壤中有害植物疗法竞争力的生物控制策略，这取决于其有吸引力的生物活性化合物的诱导的植物。此外，它们是医疗应用抗生素的有效生产者。为了鉴定这种生物的新产品，基因组采矿策略与质谱结合的是选择方法。在此，我们通过 Paenibacillus </ i>菌株进行这种研究？E681。其基因组序列的生物信息评价揭示了四种基因簇A-D编码非纤维素肽合成酶（NRPS）。因此，通过基质辅助激光解吸/电离飞行时间质谱法（MALDI-TOF MS）检测四个脂肽家族。簇？A和D编纂了众所周知的Fusaricidins和polymyxins。通过使用MALDI-LIVER-TOF / TOF MS，发现并在结构特征中发现并在结构上表征了一种未知的脂肽。它被指定为帕尼哌啶蛋白。从结构预测，我们推断出该试剂的生产由基因簇进行编码？C。基因簇？B编码三癸酸的合成，一系列开放式脂质肽肽。菌株E681产生这种化合物的新亚种（TrideCaptins？e），其显示在其脂肪酸部分以及它们的肽部分中的变化。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-16039/'>《Chembiochem: A European journal of chemical biology》</a> <b style="margin: 0 2px;">|</b><span>2018年第7期</span><b style="margin: 0 2px;">|</b><span>共10页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Vater Joachim&option=202" target="_blank" rel="nofollow">Vater Joachim;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Herfort Stefanie&option=202" target="_blank" rel="nofollow">Herfort Stefanie;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Doellinger Joerg&option=202" target="_blank" rel="nofollow">Doellinger Joerg;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Weydmann Max&option=202" target="_blank" rel="nofollow">Weydmann Max;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Borriss Rainer&option=202" target="_blank" rel="nofollow">Borriss Rainer;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lasch Peter&option=202" target="_blank" rel="nofollow">Lasch Peter;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>ZBS6 Proteomics and SpectroscopyRobert Koch-Institut BerlinSeestrasse 10 13353 Berlin Germany;</p> <p>ZBS6 Proteomics and SpectroscopyRobert Koch-Institut BerlinSeestrasse 10 13353 Berlin Germany;</p> <p>ZBS6 Proteomics and SpectroscopyRobert Koch-Institut BerlinSeestrasse 10 13353 Berlin Germany;</p> <p>ZBS6 Proteomics and SpectroscopyRobert Koch-Institut BerlinSeestrasse 10 13353 Berlin Germany;</p> <p>Fachgebiet PhytomedizinHumboldt Universit?t BerlinLentzeallee 55–57 14195 Berlin Germany;</p> <p>ZBS6 Proteomics and SpectroscopyRobert Koch-Institut BerlinSeestrasse 10 13353 Berlin Germany;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/179.html" title="分子生物学">分子生物学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=genome mining&option=203" rel="nofollow">genome mining;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=mass spectrometry&option=203" rel="nofollow">mass spectrometry;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=paenilipoheptins&option=203" rel="nofollow">paenilipoheptins;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=peptides&option=203" rel="nofollow">peptides;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=tridecaptins&option=203" rel="nofollow">tridecaptins;</a> </p> <div class="translation"> 机译：基因组挖掘;质谱;浸磷酸蛋白;肽;Tridecaptins; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li 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Genome Mining of the Lipopeptide Biosynthesis of Paenibacillus polymyxaPaenibacillus polymyxa E681 in Combination with Mass Spectrometry: Discovery of the Lipoheptapeptide Paenilipoheptin

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