Immunocytochemistry of mesenteric malignant mixed müllerian tumour in peritoneal effusion cytology: Case report

摘要

For immunocytochemistry, we employed the poly-mer-peroxidase method [Histofine? Simple Stain MAX PO (MULTI); Nichirei Biosciences, Tokyo, Japan] on centrifuged smears, without a retrieval method. We used commercially available monoclonal antibodies against cytokeratin (AE1/AE3; DAKO Cytomation, Glostrup, Denmark; diluted 1 : 200), epithelial antigen (Ber-EP4; DAKO Cytomation; diluted 1 : 100), calretinin (DAK-Calret 1; DAKO Cytomation; diluted 1 : 50), muscle actin (HHF-35; DAKO Cytomation; diluted 1 : 200), desmin (D33; DAKO Cytomation; diluted 1 : 200) and skeletal muscle myosin (Y-32; Nichirei Biosciences; diluted 1 : 1) and a polyclonal antibody against S-100 (DAKO Cytomation; diluted 1 : 3000) as described previously.