Molecular dissection of defense response of pears induced by the biocontrol yeast, Wickerhamomyces anomalus using transcriptomics and proteomics approaches

摘要

Wickerhamomyces anomalus, a yeast used for the biocontrol of postharvest diseases of fruits, significantly reduces the natural decay of pear fruit without causing any adverse changes in the pear fruit quality. In the present research, the proteome of control pear fruit and pear fruit treated with W. anomalus were analyzed by 2-dimensional electrophoresis (2-DE). Several differentially expressed proteins (DEPs) were identified in pear fruit treated with W. anomalus, among which 13 were up-regulated and 14 were down-regulated. The transcriptome of control and W. anomalus-treated pear fruits was also analyzed. RNA sequencing results revealed 1076 differentially expressed genes (DEGs) in W. anomalus-treated pear fruits, in which 51.67% (556) were up-regulated and 48.33% (520) were down-regulated (at a log(2) Fold Change >= 1, FDR < 0.05). Notably, W. anomalus induced several disease resistance-related genes and their corresponding proteins in pear fruit, as well as genes associated with the jasmonic acid signaling pathway, flavonoid biosynthesis, and carbohydrate metabolism.