OPTIMISATION OF GROWING CONDITIONS OF EUROPEAN MISTLETOE HYPERPARASITIC FUNGUS [PHAEOBOTRYOSPHAERIA VISCI): EFFECT OF DIFFERENT MEDIA AND ANTIBIOTICS

摘要

European mistletoe (Viscum album) is an evergreen, perennial, hemiparasitic shrub, which is able to infect more than 450 woody species. Besides mechanical pruning, a hyperparasitic fungus (Phaeobotryosphaeria visa) could be a successful candidate to develop an effective biocontrol agent against mistletoe. It was necessary to find antibiotics which make the isolation easier and do not effect mycelia growth negatively under strain. Furthermore, we wanted to define a medium for optimal growth under in vitro laboratory conditions. Three different media (potato-dextrose agar, modified oatmeal agar, Va PDA + V8 agar) and four antibiotics (kanamycin, ampicillin, rifampicin, nystatin) were tested. Oatmeal agar and potato-dextrose agar are optimal to maintain the fungal strains, the colony diameter was 9 cm on the 12th day. All tested antibiotics are useful, except nystatin.