QTL mapping for root vigor and days to flowering in Brassica napus L.

摘要

A segregating F-2 population was developed from a winter and spring type cross to identify quantitative trait loci (QTL) controlling root vigor and days to flowering in canola (Brassica napus). About 3090 polymorphic SNPs derived from genotyping by sequencing were used to develop a linkage map. A final linkage map was constructed with 658 SNPs at LOD 4. One QTL, NRV (Napus Root Vigor) was identified on chromosome A01 (24.7 Mbp) for root vigor explaining 16.3% of the phenotypic variation. GBF Interacting Protein 1 (GIP1) and SAUR-like family proteins are the two candidate genes related to root growth and development identified within this QTL region. Two QTL, DTF1 and DTF2, were identified for days to flowering, accounting for 21.7% and 15% of the phenotypic variation, respectively. DTF1 was assigned on chromosome C08 (9.43 Mbp) and two putative candidate genes, Light Regulated WD1 (LWD1) and FLOWERING BHLH 1 (FBH1) were identified within this QTL region. For DTF2, three putative candidate genes A. thaliana CENTRORDIALIS (ATC), Tetracopeptide Repeat (TPR), and Poly A Binding protein 3 (PAB3) were identified on the chromosome C04 (14.56 Mbp).