Fluorescence correlation spectroscopy: Analysis of molecular interactions in cell lysates [Analyse molekularer Interaktionen in Zelllysaten]

摘要

Fluorescence correlation spectroscopy (FCS) derives information on molecular interactions and concentrations from the analysis of timedependent fluctuations of a fluorescence signal, caused by diffusion of molecules through a confocal detection volume. Here, we present applications of FCS in drug discovery and molecular pharmacology, including the screening of drug-target interactions and peptide stability in crude cellular lysates, providing a physiological environment.