Methods for Staining Mitochondria and Mitochondrial Nucleoids of the Yeast Yarrowia lipolytica Grown on a Hydrophobic Substrate

摘要

The alkane-assimilating yeast Yarrowia lipolytica is an obligate aerobic yeast whose mitochondrial function is indispensable for its survival. However, little is known about the morphology of the mitochondria in Y lipolytica grown on different carbon sources. During the course of our study, we found that routine methods for staining mitochondria and the mitochondrial nucleoids of S. cerevisiae cannot be successfully applied to Y. lipolytica cells grown on a hydrophobic substrate. In this report, we present modified methods for staining these structures in Y. lipolytica cells. We also show that marked development of the mitochondria was not observed in oleic acid-grown cells, as it was in glucose-grown cells.