Lower Incubation Temperature Increases Yield of Insect Genomic DNA Isolated by the CTAB Method

摘要

Boyce et al. (1) described a simple and rapid technique to isolate genomic DNA from the pine weevil. The key step in this procedure was the incubation of pine weevil lysates in the presence of hexadecyltrimethylammonium bromide (CTAB) at 65°C. In this report, we show that incubating cell lysates at 37°C rather than 65°C yields more genomic DNA and incubation of samples at lower temperatures yields equivalent amounts of genomic DNA.