Differential loss of expression of common fragile site genes between oral tongue and oropharyngeal squamous cell carcinomas

摘要

The common fragile sites (CFSs) are large regions of profound genomic instability found in all individu-als. A number of the CFSs have been found to span genes that extend over large genomic regions (>700 kb). The expression of these genes is frequently abrogated in a number of different cancers and several of them have already been shown to function as tumor suppressor genes, both in vitro arid in vivo. We analyzed the expression of 14 large CFS genes in two distinct groups of head and neck cancersusing real-time RT-PCR. The first were oral tongue squamous cell carcinomas (SCCs) and the second were base of tongue/ton-sillar (oropharyngeal) SCCs. These two groups were previously examined for the presence of human papillomavirus (HPV) and while 46%of the oropharyngeal cancers were positive for HPV16 only one of 52 oral cancers contained HPV16 sequences. We observed a distinct pattern of loss of expression of the large CFS genes in the two groups of head and neck cancers. In addition, there was nocorrelation between the relative instability in different CFS regions and which genes were inactivated. Thus, this report demonstrates another distinction between these two groups of head and neck cancer. In addition, it suggests that there is selectionfor loss of expression of specific CFS genes in thesecancers.