Enhancement of the thermal and alkaline pH stability of Escherichia coli lysine decarboxylase for efficient cadaverine production

Kou Fengyu; Zhao Jing; Liu Jiao; Sun Cunmin; Guo Yanmei; Tan Zijian; Cheng Feng; Li Zhimin; Zheng Ping; Sun Jibin

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Enhancement of the thermal and alkaline pH stability of Escherichia coli lysine decarboxylase for efficient cadaverine production

机译：增强大肠杆菌赖氨酸脱羧酶的热和碱性pH稳定性，以高效尸培养生产

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摘要

To enhance the thermal and alkaline pH stability of the lysine decarboxylase from Escherichia coli (CadA) by engineering the decameric interface and explore its potential for industrial applications.

机译：通过工程透明界面增强来自大肠杆菌（CADA）的赖氨酸脱羧酶的热和碱性pH稳定性，探讨其工业应用的潜力。

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来源
《Biotechnology Letters》 |2018年第4期|共9页
作者
Kou Fengyu; Zhao Jing; Liu Jiao; Sun Cunmin; Guo Yanmei; Tan Zijian; Cheng Feng; Li Zhimin; Zheng Ping; Sun Jibin;
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作者单位

Chinese Acad Sci Tianjin Inst Ind Biotechnol Key Lab Syst Microbial Biotechnol Tianjin 300308 Peoples R China;

Chinese Acad Sci Tianjin Inst Ind Biotechnol Key Lab Syst Microbial Biotechnol Tianjin 300308 Peoples R China;

Chinese Acad Sci Tianjin Inst Ind Biotechnol Key Lab Syst Microbial Biotechnol Tianjin 300308 Peoples R China;

Chinese Acad Sci Tianjin Inst Ind Biotechnol Key Lab Syst Microbial Biotechnol Tianjin 300308 Peoples R China;

Chinese Acad Sci Tianjin Inst Ind Biotechnol Key Lab Syst Microbial Biotechnol Tianjin 300308 Peoples R China;

Chinese Acad Sci Tianjin Inst Ind Biotechnol Key Lab Syst Microbial Biotechnol Tianjin 300308 Peoples R China;

Zhejiang Univ Technol Coll Biotechnol &

Bioengn Hangzhou 310014 Zhejiang Peoples R China;

East China Univ Sci &

Technol State Key Lab Bioreactor Engn Shanghai 200237 Peoples R China;

Chinese Acad Sci Tianjin Inst Ind Biotechnol Key Lab Syst Microbial Biotechnol Tianjin 300308 Peoples R China;

Chinese Acad Sci Tianjin Inst Ind Biotechnol Key Lab Syst Microbial Biotechnol Tianjin 300308 Peoples R China;

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原文格式 PDF
正文语种 eng
中图分类分子生物学;
关键词
Biotransformation; Cadaverine; Lysine decarboxylase; Multimeric interface; Stability;

机译：生物转化;尸巢;赖氨酸脱羧酶;多端界面;稳定性;

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专利

1. Enhancement of the thermal and alkaline pH stability of Escherichia coli lysine decarboxylase for efficient cadaverine production [J] . Kou Fengyu, Zhao Jing, Liu Jiao, Biotechnology Letters . 2018,第4期

机译：增强大肠杆菌赖氨酸脱羧酶的热和碱性pH稳定性，以促进高效尸霉素生产
2. Characterization of a Whole-Cell Biotransformation Using a Constitutive Lysine Decarboxylase from Escherichia coli for the High-Level Production of Cadaverine from Industrial Grade l-Lysine [J] . Jihyun Shin, Jeong Chan Joo, Eunji Lee, Applied biochemistry and biotechnology, Part A. enzyme engineering and biotechnology . 2018,第4期

机译：使用组成型赖氨酸脱羧酶的全细胞生物转化的表征来自<重点型=“斜体”>大肠杆菌从工业级<重点型=“小型=”小型型“> L - 丽水
3. Coproduction of succinic acid and cadaverine using lysine as a neutralizer and CO2 donor with L-lysine decarboxylase overexpressed Escherichia coli AFP111 [J] . Wang Jing, Mao Jingwen, Tian Weilong, Green chemistry . 2018,第12期

机译：用赖氨酸作为中和剂和CO2供体与L-赖氨酸脱羧酶过表达大肠杆菌AFP111的琥珀酸和野兔用赖氨酸和CO2供体
4. H_2 Producing Activity by Escherichia Coli During Mixed Carbon Fermentation at Slightly Alkaline and Acidic pHs: Novel Functions of Hydrogenase 4 (hyf) and Hydrogenase 2 (hyb) [C] . Karen Trchounian, Armen Trchounian NATO advanced research workshop on the Black Sea: strategy for addressing its energy resource development and hydrogen energy problems . 2013

机译：H_2在略微碱性和酸性pH的混合碳发酵过程中通过大肠杆菌生产活性：氢酶4（HUF）和氢酶2（HUB）的新功能
5. Efficient experimental design and micro-scale medium enhancement of 6-deoxyerythronolide B production through Escherichia coli. [D] . Pistorino, Michael. 2008

机译：通过大肠杆菌生产6-脱氧赤藓醇B的高效实验设计和微型培养基增强。
6. High-Level Conversion of l-lysine into Cadaverine by Escherichia coli Whole Cell Biocatalyst Expressing Hafnia alveil-lysine Decarboxylase [O] . Hee Taek Kim, Kei-Anne Baritugo, Young Hoon Oh, 2019

机译：表达Hafnia alvei的大肠杆菌全细胞生物催化剂将L-赖氨酸高度转化为尸胺赖氨酸脱羧酶
7. Production of vinyl derivatives from alkaline hydrolysates of corn cobs by recombinant Escherichia coli containing the phenolic acid decarboxylase from Lactobacillus plantarum CECT 748 T [O] . Salgado, José M., Rodríguez-Solana, R., Curiel, José Antonio, 2012

机译：通过重组大肠杆菌从玉米芯的碱性水解物中生产乙烯基衍生物，所述大肠杆菌含有来自植物乳杆菌CECT 748 T的酚酸脱羧酶

Enhancement of the thermal and alkaline pH stability of Escherichia coli lysine decarboxylase for efficient cadaverine production

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