Cloning, refolding, purification and preliminary crystallographic analysis of the sensory domain of the Campylobacter chemoreceptor for multiple ligands (CcmL)

摘要

A periplasmic sensory domain of the Campylobacter jejuni chemoreceptor for multiple ligands (CcmL) has been crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 3350 as a precipitating agent. A complete data set was collected to 1.3 angstrom resolution using cryocooling conditions and synchrotron radiation. The crystals belonged to space group P2(1), with unit-cell parameters a = 42.6, b = 138.0, c = 49.0 angstrom, beta = 94.3 degrees.