Genomic sequence determination relies heavily on sequencing large numbers of plasmids from a genomic library. For plasmid isolation for high-throughput DNA sequencing using the R.E.A.L. Prep 96 Kit, cells are grown in 96-well plates (Qiagen, Valencia, CA, USA). After growth, the cells are concentrated by centrifugation and resuspended in buffer. We had difficulty resuspending the cells on the shaker of our robotic workstation (Multi-PROBE~(R) IIEX; Packard Instrument, Meriden, CT, USA) but found that they could be resuspended easily by briefly vortex mixing the 96-well plates if a small glass bead was introduced into each well first. A simple device, called a Bead-Bop, was constructed to deliver the beads into the wells. Its construction is described below,
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