Production of Lipase by Pseudomonas aeruginosa JCM5962(T) Under Semi-Solid State Fermentation: Potential Use of Azadirachta indica (Neem) Oil Cake

摘要

A widely employed strategy for increased production is to use crop residues as nutrient source in bioprocess. The plan of present work is to modify growth conditions by using byproducts of Azadirachta indica (neem) oil seed cake and optimize environmental conditions for maximum production of extracellular lipases by P. aeruginosa }CM5962(T), identified for production of extracellular lipases previously. Semi solid state fermentation medium comprising of different concentrations of Azadirachta indicaoil cake (AIOC) as substrate was studied, in which 4% of AIOC supported maximum lipase production after 96 hours of incubation at 37°C. Optimization of growth condition was done using nitrogen supplements, carbon additives and various oils. 1% Ammoniumnitrate as additional nitrogen supplement was found to be ideal parameter for improved production, whereas carbon supplements and oil additives did not have any effect. Mixed substrates of AIOC with six other oil cake residues (1:1) were also studied. Additive effect of mustard and coconut oil cake caused an increase of 29.95% and 38.26% respectively as opposed to AIOC alone. It is the first report demonstrating use of Azadirachta indica oil seed cake as fermentation medium for improved production of extracellular lipase from Pseudomonas aeruginosa JCM5962(T).