Comparative Study of Single- Use and Reusable Fermentors: Production of Recombinant Proteins Through Bacterial Fermentation

摘要

Single-use bioreactors have become widely accepted and well established for cell culture applications in the biopharmaceutical industry for over a decade (l). Abbott Diagnostics has moved into this technology already for commercial production of somebiologic molecules. However, single-use systems (SUSs) are rarely available for microbial applications, mostly because of the technical challenge in designing cost-effective SUSs that can meet high oxygen transfer needs and remove excessive heat generated during fermentation. Thus, an important part of our biologies manufacturing — antigens from microbial fermentation — still relies on conventional stainless-steel stirred tanks.