Scaling Multicolumn Chromatography to the Production Suite

摘要

LeDoux focused his presentation on downstream bioprocess techniques, specifically scaling up multicolumn processes. Relatively new advances in twin-column technology have brought significant increases in productivity over the past several years. Thatled YMC Process Technologies to adopt a twin-column approach.A challenge with traditional chromatography has been to balance purity, throughput, and recovery. "If purity is your standard," LeDoux stated, then "typically if you want high recoveries, your throughput suffers. Overloading a column reduces resolution.So if you need high recovery, you can put less on the column, and throughput drops. If I need high throughput, my recoveries will drop because I have to take smaller fractions. We need to come up with a strategy to decouple purity and recovery so that we can obtain both."