Tangential-Flow Electrophoresis

摘要

Investigation of Factors Involved in an Effective Separation of Human Serum Albumin from Human Plasma.Human plasma is a complex mixture of biomolecules such as serum albumin, immunoglobulins, coagulation factors, and others (l). These important protein biomolecules often are present at low levels or lacking in affected patients with certain life-threatening conditions. To extract those valuable proteins, a number of purification methods have been developed over time. Plasma fractionation can be traced back to the middle of the 20th century, when Edwin Cohn of Harvard University developed the first industrial process to purify proteins from crude human plasma (2). The Cohn fractionation method still is widely used today, as is a combination of several column chromatography separations and orthogonal viral inactivation or removal methods, to produce high-quality plasma-derived therapeutics (3).