P-142 CAUSES AND SOLUTIONS IN INADEQUATE THYROID FEME NEEDLE ASPIRATION SAMPLES FOR REVERSE TRANSCRIPTASE POLYMER CHAIN REACTION ANALYSIS

摘要

Reverse transcriptase polymer chain reaction (K.T-PCR) is used to detect the expression of different neopkstic markers within samples obtained from thyroid fine needle aspiration (FNA). In most published studies the number of inadequate FNA samples for RT-PCR analysis has not been evaluated. In our study we investigated the causes of inadequate thyroid FNA samples for RT-PCR analysis in attempt to find solutions for better sampling. MATERIALS AND METHODS: Inadequate FNA samples for RT-PCR analysis were defined as samples with negative GAPDH or Tg gene expression. Thyroid FNA samples of lesions with satisfactory cytologic diagnosis other than of follicuiar cell origin were excluded. A total of 332 samples for RT-PCR analysis obtained by ultrasound-guided thyroid FNA were investigated. After the aspirate was smeared for conventional cytology, the residual needle material was used for RT-PCR analysis in 215 (65%) cases. In the rest of the cases, separate puncture was undertaken to obtain material only forRT-PCR analysis. Nodules were measured in 194 (72%) adequate samples and in 28 (44%) inadequate samples. Statistical analysis was performed using the x~2 test and Student's t test. A value of p < 0.05 was considered significant.