Downregulated microRNA-155 expression in peripheral blood mononuclear cells of type 2 diabetic patients is not correlated with increased inflammatory cytokine production

摘要

Objectives: The role of miR-155 in immune responses of PBMCs of type 2 diabetic (T2D) patients has not been studied. Design and methods: 20 Healthy and 20 T2D subjects were participated in the study. miR-155 expression in PBMCs of the subjects was measured using real-time PCR. The levels of secreted IL-6 and TNF-alpha, cytokines were quantified using ELISA. Results: A downregulation of miR-155 expression was observed in untreated and LPS treated PBMCs of diabetic patients compared to controls. There was a significant upregulation of miR-155 after LPS treatment in PBMCs of both control and diabetic groups. In healthy subjects and in both untreated and LPS-treated conditions, miR-155 expression was negatively correlated with weight, waist circumference and body mass index. In diabetic group, there was a negative correlation between miR-155 expression and glucose levels only in LPS treated cells. Furthermore, systolic blood pressure was found to negatively correlate with miR-155 expression in untreated PBMCs of both healthy and diabetic subjects. The results also showed a significant correlation between miR-155 expression and TNF-alpha, and IL-6 levels in LPS treated cells. Conclusions: Our data demonstrate that miR-155 expression is reduced in PBMCs of diabetic patients and this reduced expression does not seem to be involved in increased cytokine production from PBMCs of these patients. (C) 2015 Elsevier Ltd. All rights reserved.