Caution Is Advised When cDNA Expression Libraries are Screened by Southwestern Methodologies

摘要

Sequence-specific DNA binding proteins have a central role in the molecular mechanisms that control the initiation of transcription. It is possible to clone these proteins by Southwestern screening of a cDNA expression library. However, this is a time-consuming and frustrating process. In this report, we describe two different types of cloning artifacts that investigators should be aware of before beginning a Southwestern screening of a cDNA expression library.