Modified Crush-and-Soak Method for Recovering Oligodeoxynucleotides from Polyacrylamide Gel

摘要

Oligodeoxynucleotides are often purified by polyacrylamide gel electro-phoresis (PAGE). The two commonly used methods for recovering Oligodeoxynucleotides (ODN) from polyacrylamide gels are the electroelution and the crush-and-soak methods (1). Electroelution is fast but inconvenient, as it requires a special apparatus. The crush-and-soak method is simple but time-consuming. In a standard crush-and-soak method, a gel fragment containing the ODN is excised, crushed and soaked overnight in sodium acetate buffer to extract the oligodeoxynucleo-tide. Here, we describe a modified crush-and-soak method that eliminates the overnight soaking yet gives comparable yields.