Wide-bore P-methylstyrene-co-dimethylbis(p-vinylbenzyl) silane Based-monoliths Columns for Separation of Peptides and Proteins

摘要

Wide-bore monolithic columns of p-methylstyrene-co-dimethylbis(p-vinylbenzyl)silane were prepared within glass columns (100 x 3 mm I.D.). Monolithic columns differing in the amount of initiator were fabricated. The pressure drop vs. flow-rate measurements and the relatively low swelling propensity factor indicated the good cross-linking homogeneity and good mechanically stability. The chromatographic performance of monoliths prepared was assessed by analysis of protein and peptide mixtures. Five proteins were separated in less than 2 minutes. Moreover, a mixture of 9 peptides was separated in less than 11 minutes, aside from the co-elution of vasopressin [arg8] and methionine enkephalin.