Novel beta-galactosynthase-beta-mannosynthase dual activity of beta-galactosidase from Aspergillus oryzae uncovered using monomer sugar substrates

摘要

A novel beta-galactosynthase-beta-mannosynthase dual-activity of beta-galactosidase from Aspergillus oryzae was uncovered, for the first time, using free monosaccharide substrates. The enzyme successfully converted galactose and mannose monomer sugars efficiently into Gal-beta(1-6)-Gal and Man-beta(1-6)-Man, respectively. The discovery could potentially revolutionize chemoenzymatic glycan and non-digestible oligosaccharide (NDO) syntheses.