A near-infrared BODIPY-based fluorescent probe for the detection of hydrogen sulfide in fetal bovine serum and living cells

摘要

A near-infrared (NIR) "off-on" fluorescent probe BDP-680 was developed by taking advantage of the Michael addition reaction of unsaturated double bonds in conformationally restricted BODIPY to allow detection of H2S. The new probe possesses a highly selective and sensitive response to H2S. Such a NIR probe has been applied successfully to detect H2S in 10% deproteinized fetal bovine serum (FBS). The detection limit of the probe for NaSH in 10% deproteinized FBS sample was calculated to be about 1 mu M which is consistent with the value obtained from serum free solutions (0.5 mu M). The probe is of low toxicity and has been successfully used to detect H2S in living cells.