Affinity Ranking of Phage-Displayed Peptides:Enzyme-Linked Immunosorbent Assay versus Surface Plasmon Resonance

Mojca Lunder; Tomaz Bratkovic; Gregor Anderluh

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Affinity Ranking of Phage-Displayed Peptides:Enzyme-Linked Immunosorbent Assay versus Surface Plasmon Resonance

机译：噬菌体展示肽的亲和力排名：酶联免疫吸附测定与表面等离振子共振

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Peptides with particular affinity and specificity for variety of targets are selected through panning procedure from random peptide phage display libraries.Efficiency and convenience of enzyme linked immunosorbent assay(ELISA)and surface plasmon resonance(SPR)for screening and evaluating peptide-displaying phage clones were compared using streptavidin as a model protein target.

机译：通过淘选程序从随机的肽噬菌体展示文库中选择对各种靶标具有特定亲和力和特异性的肽段。酶联免疫吸附测定（ELISA）和表面等离振子共振（SPR）用于筛选和评估展示肽的噬菌体克隆的效率和便捷性使用链霉亲和素作为模型蛋白靶标进行比较。

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《Acta Chimica Slovenica 》 |2008年第1期| 共3页
作者
Mojca Lunder; Tomaz Bratkovic; Gregor Anderluh;
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原文格式 PDF
正文语种 eng
中图分类化学 ;
关键词
ELISA; surface plasmon resonance; Biacore; peptide phage display;

机译：ELISA;表面等离子体共振;Biacore;肽噬菌体展示;

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专利

1. Affinity Ranking of Phage-Displayed Peptides:Enzyme-Linked Immunosorbent Assay versus Surface Plasmon Resonance [J] . Mojca Lunder, Tomaz Bratkovic, Gregor Anderluh Acta Chimica Slovenica . 2008 ,第1期

机译：噬菌体展示肽的亲和力排名：酶联免疫吸附测定与表面等离振子共振
2. Analysis of the Fungicide Boscalid in Horticultural Crops Using an Enzyme-Linked Immunosorbent Assay and an Immunosensor Based on Surface Plasmon Resonance [J] . Hirakawa Yuki, Yamasaki Tomomi, Harada Ayako, Journal of Agricultural and Food Chemistry . 2015 ,第36期

机译：酶联免疫吸附法和基于表面等离子共振的免疫传感器对园艺作物中的杀菌剂类杀虫剂进行分析
3. Comparative surface plasmon resonance and enzyme-linked immunosorbent assay characterisation of a monoclonal antibody with N-acyl homoserine lactones [J] . K. Wollner, X.Chen, E. Kremmer Analytica chimica acta . 2010 ,第1期

机译：具有N-酰基高丝氨酸内酯的单克隆抗体的比较表面等离子体共振和酶联免疫吸附测定法表征
4. A method to extract spots from the image of the ELISA (enzyme-linked immunosorbent assay) spot assay [C] . Chih-Yang Lin, Yu-Tai Ching, Wu-Hsieh, Engineering in Medicine and Biology Society, 2001. Proceedings of the 23rd Annual International Conference of the IEEE . 2001

机译：一种从ELISA图像中提取斑点的方法（酶联免疫吸附测定）
5. Affinity Interaction between Hexamer Peptide Ligand HWRGWV and Immunoglobulin G Studied by Quartz Crystal Microbalance and Surface Plasmon Resonance. [D] . Shen, Fei. 2010

机译：六聚体肽配体HWRGWV与免疫球蛋白G之间的亲和相互作用通过石英晶体微天平和表面等离子体共振研究。
6. Comparison of Enzyme-Linked Immunosorbent Assay Surface Plasmon Resonance and Biolayer Interferometry for Screening of Deoxynivalenol in Wheat and Wheat Dust [O] . Melanie Sanders, Daniel McPartlin, Kara Moran, 2016

机译：酶联免疫吸附法表面等离子体共振和生物层干涉法用于小麦和小麦粉中脱氧雪腐烯醇筛选的比较
7. Comparison of Enzyme-Linked Immunosorbent Assay, Surface Plasmon Resonance and Biolayer Interferometry for Screening of Deoxynivalenol in Wheat and Wheat Dust [O] . Melanie Sanders, Daniel McPartlin, Kara Moran, 2016

机译：酶联免疫吸附试验，表面等离子体共振和生物层干涉法筛选小麦和小麦粉尘中脱氧雪腐镰刀菌烯醇的比较
8. Enzyme-Linked Immunosorbent Assays for Kinins Using High-Affinity Monoclonal Kinin Antibodies. [R] . Odya, C. E., Lee, C. H. 1990

机译：使用高亲和力单克隆Kinin抗体进行酶联免疫吸附试验。

Affinity Ranking of Phage-Displayed Peptides:Enzyme-Linked Immunosorbent Assay versus Surface Plasmon Resonance

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