DIFFERENTIAL APPEARANCEOF VACUOLAR POLYPHENOLS IN BLACK PINE(PINUS NIGRA) NEEDLES IN RESPONSE TOTHE LOWERING OF SO2 IN THE AIR

摘要

The changes of vacuolar polyphenols appearance in the mesophyll cells of Black pine nee-dles during the second year of their development and the connection of these changes withdistance from the pollution source were studied as needle S-content started to decrease twoyears after the SO, concentration in the air approached the level that could be considered aslow near the Plomin thermal power plant. Investigation plot was arranged in zones: I – lessthan 10 km, II -10 to 20 km, III – 20 to 30 km around the power plant. Seven forms of vacuolarpolyphenols were detected: two forms of granules – fine and larger ones, globules, thin andthick ribbons, ribbons occupying the whole cell and homogeneous mass. In the zone I thedominating were ribbon like polyphenols. The most abundant polyphenols in the zone II wasthe globular type while in the zone III besides large and fine granules thin ribbons were,also, well abundant. According to the linkage clustering zones I and II are more similar to each other than zone III, based on the abundance of different polyphenols types. At thispoint the hypothesis that vacuolar polyphenols could be considered as some kind of "mem-ory" molecules in the environmental monitoring of the SO, effects on plants can be made.