INVESTIGATION OF FACTORS INFLUENCING THE REGENERATION EFFICIENCY OF Rubus SPECIES

摘要

An efficient regeneration system is described based on the use of several genotypes and combining different cytokinins in the regeneration process. Optimal regeneration efficiency can be obtained if the factors affecting regeneration are examined with special attention to the maintenance of the stock plants, the composition of the medium, and the pre-treatment. The maintenance of stock plants proved to be optimal if the plants were kept on modified LS medium supplemented with 0.125 mg L-1 6-benzylaminopurine (BAP) and 0.01 mg L-1 indole-3-butyric acid (IBA) in large vessels. Pre-treatment was found to increase the regeneration efficiency. Placing the leaves on to medium containing 1.5 mg L-1 BAP-riboside and 0.1 mg L-1 thidiazuron (TDZ) without wounding, and keeping them in the dark for 6 days gave the best results. The highest regeneration rate was observed on medium containing MS salts with B5 vitamins complemented with 20 g L-1 glucose, 3 mg L-1 BAP-riboside, 0.2 mg L-1 TDZ and 0.2 mg L-1 IBA. This system made it possible to achieve regeneration in each of the varieties examined, though to different extents.