Comparison of anther culture characteristics and spontaneous genome doubling in androgenic plants using maize (Zea mays L.) hybrids with varying DH line parentage

摘要

Studies were made on the haploid induction ability of hybrids originating from crosses between highly androgenic exotic doubled haploid (DH) lines and recalcitrant commercial inbreds. The results of anther induction after the first harvesting periodsuggest that all the hybrids studied in this experiment had a certain androgenic capacity; the highly androgenic DH lines are thus good sources for the introduction of haploid induction ability into non-responsive elite lines via crossing. Besides the early harvested embryos, secondary somatic embryos also provided a considerable number of plantlets. The transplantation of the plantlets into soil appeared to produce a physiological shock, resulting in a reduced number of adult plants. The spontaneous chromosome doubling rate (calculated on the basis of surviving regenerants) was very low in the genotypes examined, and only 2.4% fertile plants could be achieved from the cultured embryoids in the case of the best responding single-cross hybrid. These results indicate the necessity of an improvement in culture conditions in the plant regeneration phases and the elaboration of an efficient genome doubling technique.