Polymorphism analysis of the promoter of cow lactoferrin gene with PCR-RFLP and its correlation with subclinical mastitis.

摘要

California mastitis test (CMT) was used to detect the incidence of mastitis. 120 cows were selected and assigned into 2 groups, with 60 animals each in the control group (healthy cows) and experimental group (cows with subclinical mastitis). The relationship between subclinical mastitis and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the promoter of cow lactoferrin gene was explored. It was shown that polymorphism existed in the promoter of bovine lactoferrin gene, which suggests that this polymorphism could be associated with mastitis susceptibility.