Determination of organophosphorus aromatic nitro insecticides and p-nitrophenol by microbial-cell respiratory activity

摘要

We examined the possibility of measuring the organophosphorus aromatic nitro insecticides metaphos and sumithion as well as their hydrolysis product p-nitrophenol (PNP) by the specific respiratory activity (S RA) of Pseudomonas putida G 11, P. putida BA-11, and A cinetobacter calcoaceticum A-122. The plots of cellular SRA against the two insecticides and PNP were linear over the ranges of 0.5-2.5 mM for P. putida G I I and BA-I I and 0.5-1.0 mM for A. calcoaceticum A-122. P. putida BA-I I showed the greatest respiratory-response selectivity in the determination of the test substrates. We made comparison studies of the SRA of cells immobilised by two methods: carrier-surface adsorption and inclusion in various gels. We discuss the feasibility of developing a microbial sensor system for the determination of metaphos, sumithion, and PNP in aqueous media. (C) 2002 Elsevier Science B.V.. All rights reserved. [References: 25]