Enzymatic hydrolysis of protein wastes from carp (Cyprinus carpio) processing and the separation of hydrophobic peptides

摘要

Fish meat of carp ( Cyprinus carpio), eel (Anguilla vulgaris), trout (Salmo gairdneri), pike (Esox lucius), tench (Tinca vulgaris). white bream (Blicca bjoerkna), perch ( Perca fluviatilis), Scardinius erythrophthalmus, and Leuciscus rutilus, was hydrolyzed with the use of proteolytic enzymes. Carp meat hydrolyzate was studied in greater detail. The yield of the process was optimized with the aim to get low-molecular-weight products. The peptides obtained in this way were tested for ACE (Angiotensin Converting Enzyme) inhibitory activity. Finally the ACE inhibiting peptides were separated by chromatography on high basic anion exchanger and by hydrophobic chromatography on phenyl-Superose (Pharmacia Biotech.). Histidine containing peptides, known as an efficient remedy for gastric ulcer treatment, were separated by IMAC (Immobilized Metal-ion Affinity Chromatography) on IDA-Cu agarose (Imino-Di-Acetyl - substituted agarose saturated with cupric ion). The separation of ACE inhibiting peptides by hydrophobic interaction chromatography on phenyl-Superose was successful and the highly active peptides were obtained. IMAC on cupric ions does not separate the His-peptides, but separates all low-molecular weight peptides on the account of terminal amino groups. The method developed is believed to be useful for the laboratory scale preparation of ACE inhibiting peptides.