Label free aptasensor for Lysozyme detection: A comparison of the analytical performance of two aptamers

摘要

This work presents a comparison of two different aptamers (Apts) (COX and TRAN) for the detection of a ubiquitous protein Lysozyme (Lys) using Apt-based biosensors. The detection is based on the specific recognition by the Apt immobilized on screen printed carbon electrodes (SPCEs) via diazonium coupling reaction. The quantitative detection of Lys protein was achieved by electrochemical impedance spectroscopy (EIS). A very good linearity and detection limits for the quantitation of Lys were obtained from 0.1 to 0.8 mu M and 100 nM using Apt COX and from 0.025 to 0.8 mu M and 25 nM using Apt IRAN respectively. The obtained results showed that the developed aptasensors exhibit good specificity, stability and reproducibility for Lys detection. For real application, the aptasensors were tested in wine samples and good recovery rates were recorded in the range from 94.2 to 102% for Lys detection. The obtained recovery rates confirm the reliability and suitability of the developed method in wine matrix. The developed method could be a useful and promising platform for detection of Lys in different applications. (C) 2015 Elsevier B.V. All rights reserved.