Effect of auxin type on growth In vitro of Kiwifruit axillary buds

摘要

Kiwifruit Cultures were initiated from single-node explants on Murashige and Skoog medium containing 2 mg.l~(-1) BA. In shoot proliferation stage, 20 culture media containing MS supplemented with 3 concentrations of BA (0,1,2 mg.l~(-1)) individually and along with lAA (0,0.1,0.2 mg.l~(-1)) were compared. Maximum shoot proliferation from axillary buds was achived on media supplemented with 2 mg.l~(-1) BA alone. Formation of axillary shoots was influenced by concentration of cytokinin. In the rooting experiment, the shoots were cultured on MS medium containing BA (0,1,2,3 mg.l~(-1)) with various concentrations of IBA or lAA (0,1,2,3 mg.l~(-1)). Regenerated shoots produced prominent roots when transferred to MS medium supplemented with IBA (3.0 mg.l~(-1)). The effect of lAA on rooting was not significant. Approximately 80% of the rooted microcuttings were established successfully as container-grown plants.