HPTLC Fingerprinting Method to Distinguish Total Extract of Caralluma fimbriata from the Modified Extracts of Caralluma fimbriata

摘要

To establish the fingerprint profile of total extract of Caralluma fimbriata using high performance thin layer chromatography (HPTLC) technique. HPTLC studies were carried out. CAMAG HPTLC system equipped with Linomat V applicator, TLC scanner 3, Reprostar 3 and WIN CATS-4 software were used. HPTLC finger printing of Caralluma fimbriata leaf extract revealed that the chromalogram obtained with the reference solution shows in the middle part a Dark blue zone [Slimalumaside A] at RF 0.33 and in the upper part a Blue zone [Slimalumaside B] at RF 0.94 was observed. It can be concluded that HPTLC fingerprint analysis of total extract of Caralluma fimbriata can be used as a diagnostic tool for the appropriate identification various extracts.