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Molecular identification of a fungus associated with galls formation on Ficus cordata in the Otavi mountains of Namibia.

机译:纳米比亚奥塔维山区 Ficus cordata 上与gall形成有关的真菌的分子鉴定。

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Ficus cordata is an angiosperm tree which belongs to the family Moraceae and genus Ficus. Ficus cordata has a wide distribution in Namibia. Fig trees are very useful plants ecologically, agriculturally or economically. Figs play an important role in maintaining the ecological balance. They produce fruits which are edible to both human and animals, and they provide shelter to grazing animals. F. cordata can be planted as a living fence and its leaves are used as mulch. In addition, its bark is used for tanning skins and for dyeing hides and making. The objective of the study was to isolate and identify the fungal species associated with gall formation on the Ficus cordata in the Otavi mountains of Namibia. Fungal species were isolated from the galls on the infected twigs and grown into pure cultures using Sabouraud Dextrose Agar (SDA) and later on Malt Extract Broth (MEB). DNA was extracted from dried mycelia and conserved internal transcribed spacer regions of the fungal species were amplified using the ITS1 and ITS4 primers. DNA amplicons were sequenced and compared to sequences of known organisms in the GenBank using nucleotide BLAST (Basic Local Alignment Search Tool). The BLAST search program revealed the identity of fungal species isolated consistently cultured as an isolate of Dothideomycete species (100% similarity). However, further work of testing Koch's postulates is needed to verify whether the Dothideomycete species is directly causing the formation of galls on the Ficus cordata.
机译:无花果榕是被子植物,属于桑科和榕属。 无花果在纳米比亚分布广泛。无花果树在生态,农业或经济上都是非常有用的植物。无花果在维持生态平衡方面起着重要作用。它们产生可被人类和动物食用的水果,并为放牧动物提供庇护所。 F。可将cordata 种植成活篱笆,其叶子用作覆盖物。此外,它的树皮还可以用于鞣制皮肤,对皮革进行染色和制作。这项研究的目的是分离和鉴定与纳米比亚奥塔维山上的榕榕(Ficus cordata )的胆形成有关的真菌种类。从受感染的树枝上的胆汁中分离出真菌物种,并使用Sabouraud葡萄糖琼脂(SDA)和后来在麦芽提取液(MEB)上的纯种培养。从干燥的菌丝体中提取DNA,并使用ITS1和ITS4引物扩增真菌物种内部保守的转录间隔区。使用核苷酸BLAST(基本局部比对搜索工具)对DNA扩增子进行测序,并与GenBank中已知生物的序列进行比较。 BLAST搜索程序揭示了始终培养为 Dothideomycete 菌种(100%相似性)的分离菌种的身份。但是,需要进一步测试科赫的假设,以验证 Dothideomycete 物种是否直接导致 corcus cordata 上形成胆汁。

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