Typing of Acinetobacter baumannii strains isolated from hospital patients by amplified ribosomal DNA restriction analysis (ARDRA).

摘要

Eighty-one clinical Acinetobacter baumannii isolates were included in this study collected from year 2001 to 2006. The strains were originally isolated from different clinical and environmental specimens by the Microbiology Laboratory of the King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia. They were preserved in tryptic soy broth (TSB) supplemented with 20% (vol/vol) glycerol. Pure isolates were stored at -80 degrees C until used in the Research Centre, KFSH&RC. For ARDRA genomic typing, the 16S rRNA gene of each of the 81 Acinetobacter baumannii strains was amplified and restricted with the restriction enzymes Alu I, Mbo I and Msp I. Each enzyme generated 3-7 bands per strain, analyzed visually by comparing the differences in the banding patterns generated and grouped the isolates into Strain A to G, while isolates that are untypeable or undetermined were group into Strain H. ARDRA could not accurately distinguish the 81 different Acinetobacter baumannii strains using three restriction enzymes, indicating low discriminatory power for the method.